Identification of neutralizing epitopes on a European strain of swine vesicular disease virus.

Characterization of neutralization sites on the circulating variant of swine vesicular disease virus (SVDV): a new site is shared by SVDV and the related coxsackie B5 virus.

Using a panel of new monoclonal antibodies (mAbs), five neutralizing, conformation-dependent sites have been identified on the antigenic variant of swine vesicular disease virus (SVDV) circulating currently. In studies on the antigenic conservation of these sites, the four antigenic/genetic groups of SVDV described showed distinguishable patterns, confirming this classification. By sequencing m...

Construction of a full-length infectious cDNA clone of swine vesicular disease virus strain NET/1/92 and analysis of new antigenic variants derived from it.

The Dutch swine vesicular disease virus (SVDV) isolate NET/1/92 was one of the first isolates belonging to a new SVDV antigenic group. This strain was completely sequenced and was shown to have 93% similarity with the UKG/27/72 isolate. To enable antigenicity, replication, maturation and pathogenicity studies of NET/1/92, an infectious full-length cDNA clone, designated pSVD146, was prepared. T...

The Relative Levels of Neutralizing and Precipitating Antibodies Against Infectious Bursal Disease Virus and Their Correlation with Resistance to a Highly Virulent Challenge Strain

Mapping of linear epitopes on the capsid proteins of swine vesicular disease virus using monoclonal antibodies.

The antigenic linear map of swine vesicular disease virus (SVDV) has been studied using a repertoire of monoclonal antibodies (mAbs) raised against a recombinant SVDV polyprotein, P1. Peptide-scanning analyses, cross-reactivity studies with homologous and heterologous viruses and predicted location on a computer-generated three-dimensional model of the capsid proteins have allowed the identific...

Crude extract of bacterially expressed, infectious bursal disease virus VPX/VP2 proteins induces protective immune response to the virus in chickens

Infectious bursal disease virus (IBDV) infects young chickens and causes serious lose to the poultry industry, worldwide. Previous attempts using purified bacterially expressed IBDV VP2 failed to elicit a protective immune response to the virus. This study was designed to investigate if the initial expressed protein contained neutralizing epitopes but became nonfunctional during purification st...